Dual oxidase enables insect gut symbiosis by mediating respiratory network formation

Significance The insect respiratory system consists of tubular tracheae that transport oxygen to the organs. We show that, in pest Riptortus pedestris , establishment an essential symbiosis gut with aerobic bacterial species Burkholderia insecticola triggers development extensive tracheal network enveloping gut. Genetically blocking trachea formation prevents this symbiosis. further discovered reactive species-generating enzyme Duox is crucial for and stabilization by forming protein cross-links matrix. Reactive generated can be scavenged antioxidants such as N-acetylcysteine, feeding insects compound symbiosis, suggesting used novel insecticides.